标题: Factors influencing the removal of extracellular resistance genes in activated sludge process
作者: Xue, JY (Xue, Jinyuan); Zhang, MQ (Zhang, Mengqi); Chen, AJ (Chen, Anji); Li, YH (Li, Yuhan); Zhong, CK (Zhong, Chenke); Chen, CQ (Chen, Chaoqi)
来源出版物: BIOCHEMICAL ENGINEERING JOURNAL 卷: 221 文献号: 109791 DOI: 10.1016/j.bej.2025.109791 Published Date: 2025 SEP
摘要: Extracellular antibiotic resistance genes (eARGs) contribute to antimicrobial resistance spread in the environment, yet their removal mechanisms in activated sludge remain poorly understood. Here, an artificial resistant plasmid (PUC57-sul1) and its corresponding sul1 amplicon were treated in aerobic reactors under varying conditions. The removal of eARGs was significantly affected by sludge disinfection, biomass concentrations, aeration rate, and temperature. The results indicated that the influencing factors for eARGs are similar to those for smallmolecular-organic compounds. At optimal conditions (5 g/L biomass concentration, 400 mL/min aeration rate, and 30 degrees C), the removal efficiency at 12-h was 88.7 % for the amplicon and 67.4 % for the plasmid, respectively, indicating that eARG removal was largely affected by size and conformation. A transient increase of eARGs was observed when actual hospital wastewater was treated, likely due to the lysis of ARG-carrying bacteria. In undiluted hospital wastewater, the concentration of sul1 was reduced by 15.3 % relative to its peak concentration, even under the optimized conditions. In contrast, when the wastewater was 10-fold diluted, the concentration of sul1 was reduced by 81.3 %, confirming the effectiveness of the activated sludge process when toxicity was reduced. Microbial inhibition may be the key factor limiting eARG removal. These findings contribute to a better understanding of factors facilitating the degradation of eARGs, potentially guiding future strategies for improving wastewater treatment practices.
作者关键词: Extracellular antibiotic resistance genes; Hospital wastewater; Aerobic reactors; Influencing factors; Removal efficiency
KeyWords Plus: MUNICIPAL WASTE-WATER; ANTIBIOTICS; BACTERIA; IMPACT
地址: [Xue, Jinyuan; Zhang, Mengqi; Li, Yuhan; Zhong, Chenke; Chen, Chaoqi] Wuhan Univ, Sch Resource & Environm Sci, Hubei Biomass Resource Chem & Environm Biotechnol, Wuhan 430079, Peoples R China.
[Chen, Anji] Wuhan Britain China Sch, Wuhan 430030, Peoples R China.
通讯作者地址: Chen, CQ (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, Hubei Biomass Resource Chem & Environm Biotechnol, Wuhan 430079, Peoples R China.
电子邮件地址: [email protected]
影响因子：3.8
