标题: Engineered Microalgae-Material Biohybrid Enhances Carbon-Negative Hydrogen Production Under Emerging Contaminant Stress
作者: Wang, W (Wang, Wei); Wang, HZ (Wang, Hanzhi); Wu, QR (Wu, Qirui); Qv, M (Qv, Mingxiang); Dai, D (Dai, Dian); Huang, LZ (Huang, Li-Zhi); Zhu, LD (Zhu, Liandong)
来源出版物: ADVANCED FUNCTIONAL MATERIALS DOI: 10.1002/adfm.202519772 Early Access Date: OCT 2025 Published Date: 2025 OCT 7
摘要: In this study, a biohybrid composite (CS@PPy@Ca) is developed by encapsulating Chlorella sorokiniana (CS) with a polypyrrole (PPy) inner shell and a CaCO3 outer layer. This hierarchical coating improved structural robustness and electron transfer, enabling stable performance under stress. Under 1 mM trichloroacetic acid (TCAA), CS@PPy@Ca achieved synergistic enhancement of CO2 fixation (residual CO2 <100 ppm) and H-2 production (341.8 ppm), approximate to 12-fold higher than pristine CS (28.75 ppm) on day 3. Upon CO2 supplementation (20% on Day 3), H-2 yield increased to 420 ppm by Day 6, accompanied by a drop in residual CO2 to 90 ppm, indicating strong CO2 tolerance and high CO2 fixation efficiency under environmental stress. Transcriptomic analysis revealed upregulation of key photosynthetic genes, including those encoding photosystem II, ferredoxin, and cytochrome b(6)f complex, thereby enhancing electron transport and Calvin cycle activity. Mechanistic studies confirmed that PPy facilitated charge transfer and CaCO3 reinforced cellular integrity, jointly redirecting metabolism toward improved CO2 fixation and H-2 production. This work highlights a robust material-microbe synergy and provides a scalable strategy for CO2 valorization and sustainable hydrogen production under environmental stress.
作者关键词: CO2 fixation; environmental stressors; H-2 Production; microalgae; trichloroacetic acid
KeyWords Plus: ALGAE
地址: [Wang, Wei; Wang, Hanzhi; Wu, Qirui; Qv, Mingxiang; Dai, Dian; Zhu, Liandong] Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm Bi, Wuhan 430079, Peoples R China.
[Wang, Wei; Wang, Hanzhi; Wu, Qirui; Qv, Mingxiang; Dai, Dian; Zhu, Liandong] Wuhan Univ, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.
[Zhu, Liandong] Wuhan Univ, Natl Inst Dev Strategy, Wuhan 430072, Peoples R China.
[Huang, Li-Zhi] Wuhan Univ, Sch Civil Engn, 8 East Lake South Rd, Wuhan 430079, Peoples R China.
通讯作者地址: Zhu, LD (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm Bi, Wuhan 430079, Peoples R China.
Zhu, LD (通讯作者)，Wuhan Univ, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.
Zhu, LD (通讯作者)，Wuhan Univ, Natl Inst Dev Strategy, Wuhan 430072, Peoples R China.
Huang, LZ (通讯作者)，Wuhan Univ, Sch Civil Engn, 8 East Lake South Rd, Wuhan 430079, Peoples R China.
电子邮件地址: [email protected]; [email protected]
影响因子：19
